This study addresses the cellular uptake and intracellular trafficking of 15‐nm gold nanoparticles (NPs), either plain (i.e., stabilized with citrate) or coated with polyethylene glycol (PEG), exposed to human alveolar epithelial cells (A549) at the air–liquid interface for 1, 4, and 24 h.
Keywords: gold nanoparticle, ovarian cancer, cytotoxicity, cell proliferation, reactive oxygen species, cellular uptake, darkﬁeld imaging (Some ﬁgures may appear in colour only in the online journal) 1. Introduction Gold nanoparticles (AuNPs) are among the nanomaterials that have gained attention of researchers for various bio-medicine ...
Gold nanoparticles (GNPs) are claimed as outstanding biomedical tools for cancer diagnostics and photo-thermal therapy, but without enough evidence on their potentially adverse immunological effects. Using a model of human dendritic cells (DCs), we showed that 10 nm- and 50 nm-sized GNPs (GNP10 and GNP50, respectively) were internalized predominantly via dynamin-dependent mechanisms, and …
Influence of cell size on cellular uptake of gold nanoparticles . ... and the influence of cell size on the cellular uptake of gold NPs was investigated. Cells with a large size had a high total cellular uptake, but showed a low average uptake per unit area of cells. Cells with a small size showed opposite behaviors.
Gold nanoparticles (AuNPs) are relevant in nanomedicine for drug delivery in the vascular system, where endothelial cells are the first point of contact. We investigated the uptake of 80 nm AuNPs in primary human umbilical vein endothelial cells (HUVECs) by flow cytometry, 3D confocal microscopy, nano-scale Nanotoxicology New Talents
Uptake of Engineered Gold Nanoparticles into Mammalian Cells Lev A. Dykman † and Nikolai G. Khlebtsov * † ‡ † Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences (IBPPM RAS), 13 Prospekt Entuziastov, Saratov 410049, Russia
Jun 19, 2017· The possible mechanisms of cellular uptake for the three types of gold nanoparticles were examined, and it was found that different shapes tended to use the various endocytosis pathways in ...
How to cite this article: Johnsen KB, Bak M, Kempen PJ, Melander F, Burkhart A, Thomsen MS, Nielsen MS, Moos T, Andresen TL. Antibody affinity and valency impact brain uptake of transferrin receptor-targeted gold nanoparticles.
compositions. Gold nanoparticles have a large range of applications including electrical, biomedical, catalytic, magnetic, and optical technology [1, 13]. While this is not intended to be a comprehensive review of the current state of gold nanoparticle technology, what follows are selected examples of gold nanoparticle technology to
Engineered nanoparticles are aquatic contaminants of emerging concern that exert ecotoxicological effects on a wide variety of organisms. We exposed cetyltrimethylammonium bromide–capped spherical gold nanoparticles to wood frog and bullfrog tadpoles with conspecifics and in combination with the other species continuously for 21 d, then measured uptake and localization of gold.
Cellular uptake of nanoparticles. We determined the number of gold nanoparticles taken up by the cells using a UV-visible (UV-vis) spectroscopic method 38.According to the Beer-Lambert law, there is a linear correlation between the concentration of gold nanoparticles and the absorbance of their localized surface plasmon resonance (LSPR) peak.
The goal of my dissertation was to characterize the uptake of gold nanoparticles by the mammalian cell line A549 through determining the roles of particle characteristics and culture medium components, and by describing potential modes by which particles are endocytosed.
Apr 06, 2010· Gold plasmonic properties: the basis of their biomedical applications. Bulk gold is, of course, gold in color. But gold at the nanoscale can appear red, blue, green, or brown (Fig. 1).These colors arise as a result from interaction of conduction band electrons in the metallic nanoparticles with the electric field vector of the incident light.
Khan JA, Pillai B, Das TK, Singh Y, Maiti S (2007) Molecular effects of uptake of gold nanoparticles in HeLa cells. Chembiochem 8: 1237-1240. Kodiha M, Mahboubi H,Maysinger D(2016) Gold Nanoparticles Impinge on Nucleoli and the Stress Response in MCF7 Breast Cancer Cells. Nanobiomedicine 3: 3.
Understanding plant interactions with nanoparticles is of increasing importance for assessing their toxicity and trophic transport. The primary objective of this study was to assess uptake, biodistribution and toxicity associated with exposure of tobacco plants (Nicotiana xanthi) to gold nanoparticles …
concentration and exposure time on the uptake of AuNPs by TEM. Materials and Methods Preparation of gold nanoparticles Gold nanoparticles were prepared by the citrate reduction of HAuCl 4 following the method of Turkevich et al. . Prior to synthesis, a round …
etry, where gold nanoparticles have a distinctive absorbance spectrum. Also, for all experiments the concentrations of the gold nanoparticles were equalized before incubation with cells. In Figure 1, a plot of the number of gold nanoparticles in/on cells versus size of gold nanoparticles shows cellular uptake was heavily dependent upon the size.
Gold nanoparticles in chemotherapy and radiotherapy is the use of colloidal gold in therapeutic treatments, often for cancer or arthritis. Gold nanoparticle technology shows promise in the advancement of cancer treatments.
Eﬀect of Gold Nanoparticle Aggregation on Cell Uptake and Toxicity Alexandre Albanese† and Warren C.W. Chan†,‡,§,* †Institute of Biomaterials and Biomedical Engineering, Terrence Donnelly Centre for Cellular and Biomolecular Research, Materials Science and Engineering,
We have evaluated the uptake of 5-nm gold nanoparticles (GNPs) by liver cells and their toxicity toward liver cells, in vitro by using rat liver "precision-cut" slices (de Graaf et al., 2010) incubated with GNPs and in vivo by administering GNPs intraperitoneally to rats.
Aggregation appears to be a ubiquitous phenomenon among all nanoparticles and its influence in mediating cellular uptake and interactions remain unclear. Here we developed a simple technique to produce transferrin-coated gold nanoparticle aggregates of different sizes and characterized their uptake and toxicity in three different cell lines.
To qualitatively measure cellular uptake, gold nanoparticles can be visualized in microtomed-cell slices after exposure by transmission electron microscopy (TEM), which takes advantage of the high electron density of gold nanoparticles.
Gold Nanoparticle Uptake by Tomato Plants Characterized by Single Particle ICP-MS Author: PerkinElmer Inc. Subject: This work demonstrates the ability to track nanoparticles as they migrate from water into plants with the NexION 350 ICP-MS in Single Particle mode. Keywords
Gold Nanoparticle Size and Shape Effects on Cellular Uptake and Intracellular Distribution of siRNA Nanoconstructs Jun Yue, Timothy Joel Feliciano, Wenlong Li, Andrew Lee, Teri W Odom Chemical and Biological Engineering